HRM: an innovative technology for analysis of genetic variations – Advanced applications and critical success factors

Andreas Missel
QIAGEN GmbH, Germany


High-resolution melting (HRM) analysis is a novel technique that characterizes double-stranded PCR products based on their dissociation (melting) behavior as they transition from double-stranded DNA (dsDNA) to single-stranded DNA (ssDNA) with increasing temperature. Previously unknown and even complex sequence variations can be readily detected and characterized in an easy and straightforward way. HRM technology allows rapid and cost-effective characterization of DNA samples in emerging and challenging applications such as reliable SNP genotyping, mutation scanning, species identification and DNA methylation analysis.
In this seminar, the following topics will be discussed:
• Principles of HRM technology
• Critical success factors for High Resolution Melting Analysis
• Application Data on HRM including: SNP genotyping mutation scanning, and DNA methylation analysis.

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