Microbiomes via sequencing: Challenges and opportunities

Thomas Clavel
Junior Research Group Intestinal Microbiome, TU München, Germany

The last decades have witnessed the advent of molecular techniques for the study of complex microbial communities. In particular, high-throughput sequencing has become very popular for rapid and in-depth assessment of microbiomes in targeted or shotgun manners. In as much as these approaches delivered novel insights into many different ecosystems and open ways to so far unexplored diversity, limitations have been often neglected, especially in rapidly expending areas of research such as those around the mammalian gut microbiome. In the present talk, I will address issues about standardization and the need to go beyond single studies for global assessment of bacterial communities using 16S rRNA gene sequencing.

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