Molecular diagnostics (2)

By Michael | May 5, 2013 - 15:51 |May 31, 2013 qPCR & NGS - Freising 2013

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Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR

Steve Lefever¹, Ali Rihani¹, Filip Pattyn¹, Tom Van Maerken¹, Jan Hellemans², Jo Vandesompele^1,2 1Center for Medical Genetics Ghent, Ghent University, Ghent, Belgium; 2Biogazelle, Zwijnaarde, Belgium

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Assessment of Transcriptional Activity of Borrelia burgdorferi and Host Cytokine Genes During Early and Late Infection in a Mouse Model

Emir Hodzic University of California at Davis, United States of America

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Is lung cancer genetic heterogeneity responsible for resistance to EGFR tyrosine kinase inhibitors? Contribution of digital PCR

Pascale Tomasini, Veronique Secq, Isabelle Nanni, Antoine Carlioz, Fabrice Barlesi, L’Houcine Ouafik, Frederic Fina Assistance Publique Hôpitaux de Marseille, France

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Cost-effective real-time analysis by mediator probe (RT-)PCR

Simon Wadle¹, Stefanie Rubenwolf¹, Michael Lehnert¹, Bernd Faltin², Roland Zengerle^1,3,4, Felix von Stetten^1,3,4 1Laboratory for MEMS Applications, IMTEK – Department of Microsystems Engineering, University of Freiburg, 2Robert Bosch GmbH, Applied Research – Microsystem Technologies – Microstructuring and Assembly, Post, 3 HSG-IMIT – Institut für Mikro- und Informationstechnik, 4 BIOSS – Centre for Biological Signalling Studies, University of Freiburg

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Somatic mutations – detecting less than 0.05% KRAS and BRAF mutation in a background of wildtype DNA

Christina Andersen, Tine Y. Wollff, Alice Riva, Milo Frattini, Ulf Bech Christensen, Majbritt H. Kyneb

• Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR
• Assessment of Transcriptional Activity of Borrelia burgdorferi and Host Cytokine Genes During Early and Late Infection in a Mouse Model
• Is lung cancer genetic heterogeneity responsible for resistance to EGFR tyrosine kinase inhibitors? Contribution of digital PCR
• Cost-effective real-time analysis by mediator probe (RT-)PCR
• Somatic mutations – detecting less than 0.05% KRAS and BRAF mutation in a background of wildtype DNA

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