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Ken Livak Fluidigm Corporation, United States of America |
Abstract
Fluctuations in gene expression at the single cell level could be key for generating developmental signals and for understanding the progression of tumors. Data needs to be collected from a statistically significant number of single cells in order to determine the range of gene expression present in a population of cells. Furthermore, transcripts need to be quantified for a number of genes in order to obtain meaningful cell signatures. BioMark™ arrays provide a convenient and cost-effective system for performing multiple RNA expression assays on multiple single-cell samples. This system has been used to study single cell gene expression in embryonic stem cells, hematopoieticstem cells, cancer stem cells, and early stage embryos.
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