Unprecedented accuracy when using digital PCR

Jim Huggett 1,
1LGC & University of Surrey, United Kingdom; 

Digital PCR (dPCR) offers a unique approach for molecular anal- ysis as it counts DNA molecules, separated by limiting dilution, in a defined volume. The method offers high quantitative precision and the fact that each DNA molecule has its own minute reaction (or partition) means rare genetic variants are easier to detect. The ability of dPCR to measure accurately (with both high precision and low bias) is of particular interest as it performs absolute quan- tification and thus may offer SI traceability. This could have wide ranging implication for other methods such as qPCR and NGS. This talk will present the research into dPCR accuracy and discuss some of the implications this may have on molecular measurement both in research and more routine applications.

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