Applying the MIQE Guidelines to Screens Utilizing qPCR Focused Arrays

Gregory L Shipley
Shipley Consulting, LLC, United States of America

Performing a screening experiment utilizing qPCR and focused miRNA or mRNA plate arrays is not like running a standard qPCR experiment. Experimental design and execution require some compromises that immediately precludes the implementation of the full set of recommendations as outlined in the MIQE Guidelines. In this talk I will show the differences between the two experimental procedures and how best to setup qPCR focused array screening experiments utilizing automation. I will then discuss post-screen validation of screening results, present some tips on running these experiments and show it’s importance in completing a MIQE compliant screening experiment.

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