Digital PCR or Finding The Needle In A Haystack

Elena Grigorenko
Life Technologies, Inc, United States of America

Digital PCR is a new approach to nucleic acid detection and quantification, which allows to perform absolute quantification of targets by directly counting the number of target molecules partitioned into many individual real-time PCR reactions. The introduction of OpenArray® Real-Time PCR System to perform Taqman™ chemistry with digital PCR enable researchers to run 9,000 PCR reactions simultaneously. This approach can be used for a variety of different applications aimed on detection of rare events such as quantification of viral load in diluted plasma samples, rare allele detection and single stem cell transcript analysis. The examples of each application will be discussed. OpenArray® platform and its new digital PCR capability not only extend the portfolio of conventional PCR applications, but also provide a new, more precise answers to many different biological questions.

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