Dye spectra, dynamics and consequences

Roderic Fuerst
IT-IS Life Science, Ireland

The process of qPCR requires robust and reliable florescence monitoring of multiple dyes. Commonly qPCR systems use discrete excitation and emission filters in the monitoring of amplification and hybridization florescence. Also, algorithms used for resolving components of multiplex assays rely on the assumption that dye spectra remain constant during PCR and subsequent melting point analysis. In some cases this assumption is not valid. The nature and consequences of different approaches will be discussed.

Back to MIQE and QC strategies in qPCR
Bookmark the permalink.

Comments are closed.