Extracellular vesicle subsets and sampling strategies for nucleic acid profiling

Kenneth W. Witwer 1,
1Johns Hopkins University School of Medicine, United States

Enthusiasm continues unabated for the clinical potential of extracellular RNA biomarkers, especially those associated with extracellular vesicles (EVs) that may be traced back to a cell of ori- gin. At the same time, it has become apparent that a diversity of EVs are produced by cells under normal and pathological conditions. In this presentation, data from three “challenging biofluid studies” are shared to demonstrate the influence of sampling strategy on EV subsetting and nucleic acid profiling results. Specifically, samp- ling site (for blood) is shown to affect recovery of large EVs; blood additives substantially influence small RNA profiling results; and different sampling strategies for cervicovaginal secretions affect interpretation of small RNA content by qPCR and qPCR array.

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