Uncovering the Diversity of Individual Cells: Gene Expression Profiling with the BioMark System

Ken Livak
Fluidigm Corporation, United States of America

Fluctuations in gene expression at the single cell level could be key for generating developmental signals and for understanding the progression of tumors. Data needs to be collected from a statistically significant number of single cells in order to determine the range of gene expression present in a population of cells. Furthermore, transcripts need to be quantified for a number of genes in order to obtain meaningful cell signatures. BioMark™ arrays provide a convenient and cost-effective system for performing multiple RNA expression assays on multiple single-cell samples. This system has been used to study single cell gene expression in embryonic stem cells, hematopoieticstem cells, cancer stem cells, and early stage embryos.

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