Forensic application of microbiome profiling

Lisa-Marie Link 1,2, Jens Söchtig2, Irmgard Riedmaier-Sprenzel2, Burkhard Rolf 2,*,
1 Eurofins Medigenomix GmbH, Germany
2 Eurofins Medigenomix Forensik GmbH, Germany 

The microbiome is defined as the collective genomes of the microbes that live inside and on the human body. In a forensic context, this is the collective genomes of the microbes that are present in a stain sample. Investigating such samples with human specific STR-markers ignores the information that might be present in this part of the extracted DNA. A forensic application could either use this for (additional) identification of the stain donor or for the investigation of the source level of a stain. We investigated the microbiomes of skin, mouth, nose and vaginal swabs from a set of volunteers by V4 16S ribosomal RNA massive parallel sequenc- ing. Results of this study show the great diversity of the microflora. The species observed by us in the various sample types overlap to some extent. Potential applications of the data are discussed.

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